microneedle biosensor
Figure 4a shows the sequential addition of different glucose concentrations into a phosphate buffer solution, and the current signal increases as the glucose concentration increases. Tackling the challenges of developing microneedle-based electrochemical sensors. Recent advances in the field of microneedle devices are having an impact on both diagnostic and therapeutic approaches to sustaining healthy populations globally. Multifunctional structural color triboelectric microneedle patches for 17 mg/L. Infusion pressure and pain during microneedle injection into skin of human subjects. Copyright 2019 Authors, licensed under a Creative Commons Attribution 4.0 License. d Calibration curve for the detection of glucose in goat plasma. A universal material testing machine (MTS SYSTEMS (China), Co., Ltd., China) was used to study the mechanical strength of microneedles under compression testing. Microneedle biosensors for real-time, minimally invasive drug b Cyclic voltammograms of the sensor after the deposition of 1 and 8 cycles of Prussian blue. Whichever analyte is targeted, it is imperative that consideration is given to the difference, if there is one, between ISF and blood plasma concentrations. Reproduced with permission from Gowers et al., ACS Sens. Simultaneous detection of glucose, uric acid and cholesterol using flexible microneedle electrode array-based biosensor and multi-channel portable electrochemical analyzer. Forlenza, G. P. et al. Amer Diabet, A. Pharmacologic approaches to glycemic treatment: standards of medical care in diabetes-2020. PubMedGoogle Scholar. 14, 8898 (2018). Credit: Imperial College London / Timothy Rawson et al. The measurement repeatability of this sensor for detecting glucose was studied, as shown in Fig. As shown in the figure, although there are small current changes caused by the interferences, these signals are small enough compared to those generated by blood glucose or ISF glucose. The microneedle array was placed on the bottom circular plate workbench. There have been several commercial subcutaneous glucose sensors for continuous monitoring of diabetes, such as G6 (Dexcom, San Diego, USA)22, Freestyle Libre (Abbott, Chicago, USA)23, and Guardian (Medtronic, Northridge, USA)24. volume7, Articlenumber:75 (2021) Microneedle biosensors for real-time, minimally invasive drug monitoring of phenoxymethylpenicillin: a first-in-human evaluation in healthy volunteers Lancet Digit Health. 16, 377390 (2020). Actuat. Figure 3b shows the study of this device selectivity by comparing the signals from the interferences and glucose. 29, 100348 (2020). Briefly, an MN-based sensing device consists of micron-sized arrays that are arranged on a miniature patch in a specific order. Integrated hollow microneedle-optofluidic biosensor for - Nature The sensor shows a linear detection range from 0.8 to 24mM with a slope of 0.04160.0028A/mM. Ther. 11, S17S24 (2009). Figure 4f shows the calibration curve for detecting glucose. Published by Elsevier Ltd.. All rights reserved. Microdialysis requires insertion of a needle and a hollow fiber under the skin, causing some discomfort and necessitating the wearing of a peristatic pump and collecting discrete samples. The working electrode lead from the potentiostat was connected to the Au working electrode of the sensor. The difference between the height of the microneedle and the insertion depth may be attributed to the elastic deflection on the skin during microneedle insertion, resulting in part of the microneedle remaining outside the skin44,45. Each error bar was from three sensors. f Correlation between the subcutaneous glucose levels measured by the microneedle biosensing device and the blood glucose levels measured by a commercial glucose meter for a normal mouse after being injected with glucose. Bookshelf A working electrode potential of 0.6V versus Ag/AgCl was used for detecting H2O2. Recent Advances in Microneedle-Based Sensors for Sampling, Diagnosis and Monitoring of Chronic Diseases by zgecan Erdem 1,, Ismail E 1,, Garbis Atam Akceoglu 1, Yeeren Saylan 2 and Fatih Inci 1,3,* 1 UNAMNational Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey 2 A thinner Prussian blue layer could provide a wider measurement range, which was essential for detecting a high glucose level in the ISF. B. Nguyen, T. T. & Park, J. H. Human studies with microneedles for evaluation of their efficacy and safety. The current value on each day was recalibrated according to its relative response over 7 days (Fig. 85, 1826 (2018). During diabetes management, insulin is frequently used. J. Endocrinol. Zhang, Y. et al. Using chlorination, the Ag layer became an Ag/AgCl layer, acting as the reference/counter electrode of the sensor. Sci. D. M. E. Freeman, A. E. G. Cass; A Perspective on microneedle sensor arrays for continuous monitoring of the body's chemistry. A wide and varied array of enzymes are applicable for use in biosensing devices. Published by Elsevier Ltd. The microneedle array was fabricated by 3D printing with a clear resin. Commercial glucose meters (Sinocare Inc., China) were used to compare the detection results. They require some medical training to reliably collect as well as laboratory instrumentation to analyze.3 Perhaps most importantly, information about time dependent changes in their concentrations are lost in the interval between collection and analysis. 128, 337343 (2015). Li, X. L. et al. 2007 Dec;45(12):669-76. doi: 10.5414/cpp45669. Under the plasma environment, 50l of goat plasma (Senber Biotechnology Co., China) was dropped onto the sensor, and 2l of different concentrations of glucose were added incrementally onto the working electrode. Many approaches to making microneedles for sensing have been described in the literature, covering both materials, geometries, and fabrication methods. In the in vitro penetration test, the section of the puncture in mouse skin shows that the penetration depth was approximately 0.5mm (Fig. For comparison, a linear curve was plotted between the sensing results from the microneedle biosensing device and the detection results from the commercial blood glucose meter. Solution: PBS containing 5mM H2O2. In terms of the measured glucose concentration of 5mM, it is small enough, and the ability to quantify ISF glucose is sufficient. The studied glucose concentration range corresponded to a typical range of blood glucose for normal and diabetic patients. 3e). Diabetes Care 43, S98S110 (2020). Microneedle biosensor: A method for direct label-free real time protein Gowers SAN, Freeman DME, Rawson TM, Rogers ML, Wilson RC, Holmes AH, Cass AE, O'Hare D. ACS Sens. An official website of the United States government. Eur. Outputs were captured as sensor current and compared with lactate concentrations from venous blood and microdialysis. An in situ nanomechanical test system (TI-900 TriboIndenter, Hysitron, Ltd., USA) was used to extract the elastic modulus and hardness of the microneedle. a Current versus time curve upon the addition of glucose in PBS. Glucose concentrations in parotid fluid and venous blood of patients attending a diabetic clinic. Under this layer, the viable epidermis (all other layers of the epidermis) is composed of various cell types and then beneath that the dermis with its capillary bed. A decrease in the local pH caused by an increased concentration of acid within a -lactamase containing hydrogel layer shifts the equilibrium between IrIII and IrIV in the IrOx layer. The working or reference/counter electrode area has a length of 5.2mm and a width of 1.6mm, with 27 microneedles in 9 rows and 3 columns. Blood glucose was measured at the time indicated by the arrow by using a commercial blood glucose meter. The safety of microneedles has been the focus of concern for researchers. 99, 42284238 (2010). School of Materials Science and Engineering, Peking University, 100871, Beijing, P. R. China, Renal Division, Peking University Institute of Nephrology, Peking University First Hospital, 100034, Beijing, P. R. China, You can also search for this author in 17, 787794 (2015). Timely calibration may eliminate the small errors caused by changes in the environment and the activity loss of GOD. g An EDS point analysis of the working electrode in the part of a microneedle. Figure 1h, i show the EDS mapping of the Ag/AgCl electrode, which demonstrates that the Ag and Cl elements were evenly distributed on the sidewalls of the microneedles. Wearable Clinic: From Microneedle-Based Sensors to Next-Generation Healthcare Platforms. and Y.C. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Chem. Ura T, Kagawa A, Sakakibara N, Yagi H, Tochio N, Kigawa T, Shiraki K, Mikawa T. Sci Rep. 2023 Jan 25;13(1):1435. doi: 10.1038/s41598-023-28040-1. The detection results were highly correlated with those obtained from a commercial blood glucose meter. For both diabetic patients and normal people, ascorbic acid usually has a concentration of <0.1mM in serum46,47, and uric acid usually has a concentration ranging from 0.1 to 0.4mM in serum48. f Calibration curve for the detection of glucose in simulated interstitial fluids. The new sensor relies on the coupling of the effective biocatalytic action of organophosphorus hydrolase (OPH) with a hollow-microneedle modified carbon-paste array electrode transducer, and involves rapid square-wave voltammetric (SWV) measurements of the p . The major interfering substances in the blood and ISF are ascorbic acid, uric acid, and lactate. Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). Microneedle array sensor for monitoring glucose in single cell using glucose oxidase-bonded polyterthiophene coated on AuZn oxide layer. Despite the recent progress made in microneedle-based biosensors, their current reliance on laborious sample processing or electronic hardware makes them poorly suited for use in resource-limited . Fluorescent-based biodegradable microneedle sensor array for - Science American Crystallographic Association, Inc. AVS: Science and Technology of Materials, Interfaces and Processing, https://doi.org/10.1038/s41587-019-0040-3, https://doi.org/10.1016/j.tibtech.2020.03.001, https://doi.org/10.1080/1061186X.2016.1200589, https://doi.org/10.1016/j.biopha.2018.10.078, https://doi.org/10.1016/j.addr.2012.04.005, https://doi.org/10.1519/JSC.0000000000002122, https://doi.org/10.1016/j.sna.2011.04.028, https://doi.org/10.1007/s40279-019-01158-x, https://doi.org/10.1038/s41746-019-0150-9, https://doi.org/10.1016/j.bbrep.2015.11.010, https://doi.org/10.1016/j.biotechadv.2009.04.003, https://doi.org/10.1016/j.bios.2017.01.016, https://doi.org/10.1007/s00216-016-9961-6, https://doi.org/10.1016/j.coelec.2019.04.003, https://doi.org/10.1016/j.sbsr.2016.10.004, https://doi.org/10.1177/193229681200600239, https://doi.org/10.1021/acssensors.9b00288, https://doi.org/10.1016/S2589-7500(19)30131-1, https://doi.org/10.3390/pharmaceutics9040043, https://doi.org/10.1007/s11095-014-1335-1, https://doi.org/10.1038/s41541-019-0134-4, https://doi.org/10.1016/j.snb.2021.129447, https://doi.org/10.1007/s13300-018-0436-y, http://creativecommons.org/licenses/by/4.0/, Impurities and defects in 4H silicon carbide, Hot electron transport and impact ionization in the narrow energy gap, Multi-element metasurface system for imaging in the near-infrared, Polyglycolic acid microneedles modified with inkjet-deposited antifungal coatings, Solid and hollow metallic glass microneedles for transdermal drug-delivery, Development of cup shaped microneedle array for transdermal drug delivery, Modification of microneedles using inkjet printing, Novel tungsten oxide microneedles with nanosized tips. Res. 2011, 604715 (2011). 2015 Feb 15;64:138-46. doi: 10.1016/j.bios.2014.08.072. Measurement of the composition of an individual's biofluids forms the basis of much contemporary clinical diagnostic science. J. Caring Sci. 3f). Sci. e An SEM image of 3D printed cone-shaped microneedles with a base diameter of 400m and a height of 1.5mm fabricated by using MoonRay (SprintRay Technology Ltd., China) and clear light-sensitive resin. Liu, Y., Yu, Q., Luo, X. et al. Review of a commercially available hybrid closed-loop insulin-delivery system in the treatment of Type 1 diabetes. To characterize this effect in our context, we measured and compared the biosensor response to tobramycin (10 M) before and after repetitive insertions into a piece of porcine skin. 1l). You are using a browser version with limited support for CSS. Furthermore, we verified the sensing performance in a simulated ISF. Continuous Measurement of Lactate Concentration in Human Subjects through Direct Electron Transfer from Enzymes to Microneedle Electrodes. Diabetes Technol. c Cyclic voltammograms of the sensor after the deposition of 8 cycles of Prussian blue. Bandaru, P. & Shankar, A. l An optical image of the pierced skin with staining after removing the microneedle array. Clipboard, Search History, and several other advanced features are temporarily unavailable. The authors thank the following funding agencies for supporting our work described in this paper: the National Institute for Health Research (NIHR)'s Invention for Innovation (i4i) Programme (Grant Ref. Data sharing is not applicable to this article as no new data were created or analyzed in this study. The site is secure. J. Diabetes Res. b A schematic illustration of the microneedle array. Microneedle patch for the ultrasensitive quantification of - Nature Generally, when the potential value is larger, the oxidation or reduction rate of H2O2 is higher, and the current value is larger. Careers. The abdominal skin of the mouse was used as the model to investigate whether the microneedle array could be inserted into the skin. Diabetes is among the most prevalent and challenging diseases of the twenty-first century. One limitation of the oxidase/hydrogen peroxide approach is that at the working potential for hydrogen peroxide oxidation other endogenous and exogenous molecules are oxidized. Most of the published work to this point has utilized enzymes as the recognition element of solid microneedle sensors so the focus is in this area. Health care 2020: reengineering health care delivery to combat chronic disease. Furthermore, a Prussian blue (including 2.5mM FeCl3, 100mM KCl, 2.5mM K3Fe(CN)6, and 100mM HCl) mediator layer was deposited onto the Au working electrode by cyclic voltammetry from 0.15 to 0.3V (vs. Ag/AgCl) for 8 cycles at a rate of 20mV/s. Karpova, E. V. et al. d Current versus time curve of the biosensing device for detecting subcutaneous glucose in a normal mouse after being injected with glucose. A diluted glutaraldehyde (2%) was mixed with GOD (Toyobo Ltd., Toyobo) solution (50U/l) at a volume ratio of 1:1, and then 20l of the mixture was deposited on the Au working electrode. Minimally invasive microneedle biosensors are cost-effective, can perform self-testing analyses while avoiding sweat collection. Fried, D. A. Chem. Mater. l Clark error grid for the microneedle biosensing device. These concentrations are low enough and would not affect glucose sensing. 2022 Nov 3;189(11):440. doi: 10.1007/s00604-022-05510-3. Silk/polyols/GOD microneedle based electrochemical biosensor for a Schematic illustration of the preparation process for the biosensor. A potentiostat (A CHI660e, from CH Instruments, China) was used with a constant voltage of 0.6V at the Au working electrode (versus the Ag/AgCl electrode) to measure the currents of the device under different circumstances. This is to mimic the situation of insulin treatment for diabetic patients. https://doi.org/10.1038/s41551-020-00650-4 (2020). It is a self-renewing tissue composed of three primary layers: the epidermis, the dermis, and the hypodermis. Wang, Z. et al. i Correlation between the subcutaneous glucose levels measured by the microneedle biosensing device and the blood glucose levels measured by a commercial glucose meter for a diabetic mouse after being injected with insulin. Front Bioeng Biotechnol. HHS Vulnerability Disclosure, Help 52072007, No. a A camera image of the microneedle array applied to a mouse. H2O2 was added onto the sensor, diffused to the surface of the working electrode, and then oxidized to produce a current signal. Furthermore, the signals from this device are compared with those from a commercial blood glucose meter to obtain its accuracy for diabetes monitoring. Diabetes Care 36, 793800 (2013).
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