pcr blunt ii topo invitrogen
Can be used to generate nested deletions for sequencing. This is a free resource for the scientific community that is compiled by Addgene. Sequence Author: Thermo Fisher (Invitrogen), SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. The pCRBlunt II-TOPO vector used in this kit comes with lethal ccdB gene for efficient selection of blunt-ended PCR recombinants products generated by thermostable proofreading polymerases. Le kit de clonage de PCR Zero Blunt permet de cloner facilement et efficacement (>80%) les produits de PCR aux extrmits franches, qui ont t amplifis par des ADN polymrases thermostables activit correctrice. no.K2800-02) is shipped with an additional box containing reagents for plasmid purification (Box 3). Blunt DNA Cloning Vectors, TOPO Cloning Vectors, pCR, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool, Chemical Storage and Management Resource Center, EcoRI sites flanking the PCR product insertion site for easy excision of inserts, Kanamycin and Zeocin resistance genes for your choice of selection in, SP6 promoter/primer site for in vitro RNA transcription and sequencing, M13 forward and reverse primer sites for sequencing or PCR screening, Fast and easy: go from PCR to clones in just three steps and in as little as 5 minutes hands-on time, Efficient: obtain up to 95% clones with correct insert, Proven: reliable performance for over a decade with over 4,000 citations, Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences are required, Vector: pCRBlunt II-TOPO vector: contains the, Cloning method: TOPO cloning: Topoisomerase Ibased, 5-minute ligation of blunt-ended PCR products amplified with proofreading thermostable polymerases, Competent cells: Various options: choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells (available separately) or use your own, Antibiotic resistance: Ampicillin (AmpR), Kanamycin (KanR). Backbone Vector backbone pCR-Blunt II-TOPO (Search Vector Database) Backbone manufacturer Invitrogen Vector type Mammalian Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 g/mL Growth Temperature 37C Kit includes linearized and topoisomerase I-activated pCR-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase. Does Addgene accept orders by fax, phone or email? What is virus associated DNA, and why do I have to order it. However, we are committed to improving your shopping experience. pCR-Blunt II-TOPO (linearized) Sequence and Map - SnapGene pCRBlunt II-TOPO vector: designed for proofreading polymerasesThe pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase. Therapeutic homology-independent targeted integration in retina and Bioz Stars score: 86/100, based on 1 PubMed citations. I have even tried to increase the insert (2:1 . Search Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. 2. Privacy Policy. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Le kit de clonage de PCR Zero Blunt sappuie sur le vecteur de clonage multifonctions PCR-Blunt et la ligase dADN ExpressLinkT4 de faon gnrer temprature ambiante et en cinq minutes un produit de ligature. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Zero Blunt TOPO PCR Cloning Kit, with pCR-Blunt II-TOPO Vector, One Shot TOP10 Chemically Competent, Spectroscopy, Elemental and Isotope Analysis. PDF Zero Blunt TOPO PCR Cloning Kit - Thermo Fisher Scientific This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. PDF TOPO TA Cloning - Molbio Eco R I sites flanking the PCR product insertion site for easy excision of inserts. Have questions about your order, deposit, or a plasmid? The quality of . This website uses cookies to ensure you get the best experience. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7kPlasmids:I.M.A.G.E. However, we are committed to improving your shopping experience. USER GUIDE Zero BluntTOPO PCR Cloning Kit For 5-minute cloning of blunt-end PCR products Catalog Numbers K2800-20, K2800-40, K2800-J10, K2820-20, K2820-40, K2830-20, K2860-20, K2860-40, K2800-02, 450245 Revision C.0 Publication Number MAN0000062 For Research Use Only. Receive the latest news, hot plasmids, discounts and more. Please sign in to view account pricing and product availability. Includes the Zero Blunt TOPO vector containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. What strain of bacteria does my stab contain? Speed is less than 5000g. For Research Use Only. Please use the form below to provide feedback related to the content on this product. These products typically do not have pictures or detailed descriptions. be delivered, each vial is about 5g. Try SnapGene for Free. Thermo Fisher Scientific. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. If you run into any problems registering, depositing, or ordering please contact us at [emailprotected] Learn more, Please note: Your browser does not fully support some of the features used on Addgene's website. Addgene - Analyze Sequence Welcome to Vector Database! Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Zero Blunt TOPO PCR Cloning Kit for Subcloning, without competent cells, EcoRI sites flanking the PCR product insertion site for easy excision of inserts, Kanamycin and Invitrogen Zeocin resistance genes for your choice of selection in, M13 forward and reverse primer sites for sequencing or PCR screening, Flexibility to use your own competent cell strain, Electrocompetent cells for highest transformation efficiency, Includes PureLink Quick Plasmid Miniprep Kit. pCR4Blunt-TOPO (linearized) Sequence and Map - SnapGene Learn about the latest plasmid technologies and research tools. The pCRBlunt II-TOPO vector contains: The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. Does Addgene accept orders by fax, phone or email? The pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase. | Download SnapGene Viewer. The Zero Blunt PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. I have used 4 ul of salt. Contact Addgene. ("TOPO" Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector. Resistant to Kanamycin (KanR) and Zeocin (ZeoR). Introduction Follow these instructions to TOPO Clone your blunt-end PCR product into pCR-Blunt II-TOPO and transform the reaction into chemically competent E. coli cells. Today, Invitrogen TOPO PCR cloning kits are still among the fastest, simplest, and most flexible cloning solutions available. no. Has anyone grown pCR-BluntII Topo in-house? | ResearchGate Not for use in diagnostic procedures. Explore Over 2.7k Plasmids: I.M.A.G.E. pCR-Blunt II- K n TOPO in 3519 bp site: 1099-1236 bases gene bases sit ORF:586-888: bases406-425 bases 433-448 1237-2031 Zeocin Learn more . Genome Each Zero Blunt TOPO for subcloning Kit contains linearized and topoisomerase I-activated pCR Blunt II-TOPOVector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent or ElectrocompE. coli, S.O.C. Have questions about your order, deposit, or a plasmid? We will not share your information for any other purposes. Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier Structured Review Thermo Fisher pcr blunt ii topo backbone vector Pcr Blunt Ii Topo Backbone Vector, supplied by Thermo Fisher, used in various techniques. Subscribe to Our Blog. We will not share your information for any other purposes. Editing, Cloning & ORFs. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. PCR-Blunt II-Topo kit) and incubate at 37C with shaking for 1 hr. |, Publications If you run into any problems registering, depositing, or ordering please contact us at [emailprotected] Efficient cleavage requires at least two copies of the BpmI recognition sequence. PDF QUICK REFERENCE Zero Blunt TOPO PCR Cloning Kit - Fisher Sci Zero Blunt TOPO PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. Therapeutic homology-independent targeted integration in retina and This vector is NOT available from Addgene. For transformation of electrocompetent cells, a diagram of the multiple cloning site, and a manual, see www.lifetechnologies.com/support or contact Technical Support. Not for use in diagnostic procedures. Exceptional. Consortium Plasmids Source/Author: Invitrogen (Life Technologies) Selection Marker: Neomycin/G418 (Geneticin) Copy Number: Zero Blunt TOPO PCR Cloning Kit, without competent cells We appreciate your feedback. & ORFs. For NGS library preparation, 10 ng of DNA from the PCR products were used as input for the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). PDF CRISPR/Cas9-Directed Genome Editing of Cultured Cells - Harvard University For Research Use Only. Medium, and a supercoiled control plasmid. Each kit has the Zero Blunt TOPO vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants so you can obtain up to 95% clones with correct insert. Learn about the latest plasmid technologies and research tools. pCR-Blunt II-TOPO (linearized) Linearized vector for TOPO cloning of blunt PCR products. What do I need to know about the customs and importation process for my country? Parental sequencing vector for TOPO TA cloning of PCR products. Not for use in diagnostic procedures. Search Medium are not provided. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Consortium Plasmids|More Plasmid Sets No matches HomePlasmidsI.M.A.G.E. Learn about the latest plasmid technologies and research tools. Learn more, Download our file to copy and paste plasmid data, Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments, Basic analysis for a user-entered sequence; includes restriction sites and map, Digital collection of empty plasmid backbones from publications and commercially available sources. PDF Zero Blunt PCR Cloning Kit - Thermo Fisher Scientific This is a free resource for the scientific community that is compiled by Addgene. What strain of bacteria does my stab contain? SP6 promoter/primer site for in vitro RNA transcription and sequencing. Please use the form below to provide feedback related to the content on this product. Privacy Policy. Genome Simple. The pCR Blunt II-TOPO vector features: Contains the ccdB gene for positive selection; EcoRI sites flanking the PCR product insertion site for easy excision of inserts; Kanamycin and Invitrogen Zeocin resistance genes for your choice of selection in E. coli; SP6 promoter/primer site for in vitro RNA transcription and sequencing; M13 forward and reverse primer sites for sequencing or PCR screening Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. This website uses cookies to ensure you get the best experience. The kits include linearized and topoisomerase I-activated pCR-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase (1). Spread 100 l of the transformation mixture . Receive the latest news, hot plasmids, discounts and more. Analyze Sequence: pCR blunt II TOPO. Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. Room 229, Building 26, No. Zero Blunt TOPO PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. K2800-20) including One Shot Top10 Chemically Competent E. coli cells (other competent cells for cloning may also be used) Sterilized glass beads (EMD Millipore, cat. Get the cloning results you can count on Twenty years ago, Invitrogen TOPO technology became known for superior quality and game-changing innovation in cloning. Close the tube and incubate for 10 minutes at room temperature. Systems, Research Zero Blunt PCR Cloning Kit - Thermo Fisher Scientific Your feedback has been submitted. How can I be notified when a plasmid from a specific lab or paper is available? no. Thermo Fisher Scientific, Save time by having your items shipped automatically. The maps, notes, and annotations in the zip file on this page are copyrighted material. How do I place an order? The pCRBlunt II-TOPO vector contains: The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. PCR products were cloned into PCR-Blunt II-TOPO (Invitrogen) and single clones were sequenced to confirm the identity of the PCR products before NGS analysis. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. I am trying to clone this insert in PCR BLUNT end TOPO vector. & Engineering, Model Citing NovoPro. The Zero Blunt TOPO PCR Cloning Kit for Subcloning, without competent cells allows the flexibility of using your own strain for increased flexibility so competent cells and S.O.C.
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